Suboptimal Primary Antibody Incubation

Symptom

Inconsistent or weak staining results despite following protocol. Staining intensity varies between experiments using same antibody lot and tissue type.

1. 1 Primary antibody incubation time shorter than validated protocol (CST antibodies validated for overnight at 4°C)
2. 2 Incubation temperature not controlled or inconsistent
3. 3 Deviation from rigorously tested, product-specific protocol

1. 1 Follow CST validated protocol: incubate primary antibody overnight at 4°C for optimal, consistent results
2. 2 Maintain consistent incubation temperature (4°C) across all experiments
3. 3 Refer to product-specific datasheet for rigorously tested incubation conditions; do not modify without re-validation

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