Migration is too slow — treatment and control look identical (Wound Healing Assay)

Question

Accepted Answer

After extended culture the wound has barely closed and the difference between groups is not visible. Common causes: 1) Cell vitality is poor 2) Culture conditions are unsuitable 3) Observation window is too short 4) Serum level is too low or chemotactic stimulation is inadequate Solutions: 1) Check cell state (passage, confluence, mycoplasma) 2) Extend the observation window appropriately 3) Optimize serum concentration and treatment conditions for the cell line