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Wound Healing Assay moderate

Scratch is too wide or uneven

Symptom
Scratch edges are rough and the width varies along the line, making it impossible to accurately compare migration distances later.
Common Causes
  1. 1 Pressure on the pipette tip is uneven
  2. 2 Different scratch tools used across wells
  3. 3 Monolayer confluence is not uniform
Solutions
  1. 1 Use the same model of pipette tip — or better, a dedicated scratch tool
  2. 2 Move in a single straight line at constant speed
  3. 3 Plate evenly and confirm consistent monolayer before scratching
Related Video (2)
YouTube (Curated Tutorials) ★ 85
Scratch Assay Tutorial
"Directly teaches scratch assay technique and would demonstrate proper pipette tip handling to avoid uneven pressure and rough edges"
YouTube (Curated Tutorials) ★ 80
Wound healing
"Covers wound healing assay setup and execution, providing visual instruction on correct scratch creation methodology"
Source: xiaohongshu.com ↗
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