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Wound Healing Assay moderate

Scratch is too wide or uneven

Symptom
Scratch edges are rough and the width varies along the line, making it impossible to accurately compare migration distances later.
Common Causes
  1. 1 Pressure on the pipette tip is uneven
  2. 2 Different scratch tools used across wells
  3. 3 Monolayer confluence is not uniform
Solutions
  1. 1 Use the same model of pipette tip — or better, a dedicated scratch tool
  2. 2 Move in a single straight line at constant speed
  3. 3 Plate evenly and confirm consistent monolayer before scratching
Related Video (2)
YouTube (Curated Tutorials) ★ 85
Scratch Assay Tutorial
"Directly demonstrates the scratch assay technique, including how to make an even, consistent scratch."
YouTube (Curated Tutorials) ★ 70
Wound healing
"Shows setup and analysis of wound healing assay, useful for understanding proper technique to avoid uneven scratches."
Source: xiaohongshu.com ↗
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