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Western Blot (Sigma Protocol) minor

Non-parallel Bands

Symptom
Protein bands appear curved, wavy, or not parallel to each other
Common Causes
  1. 1 Gel has not polymerized evenly or parallel
Solutions
  1. 1 Review gel preparation procedure to ensure even polymerization
  2. 2 Ensure casting apparatus is level during gel polymerization
Related Video (3)
Cell Signaling Technology ★ 88
Western Blot Troubleshooting Guide
"Dedicated Western blot troubleshooting guide directly addresses diagnosis of band appearance issues like uneven/non-parallel bands."
Bio-Rad Laboratories ★ 72
Western Blotting
"Demonstrates SDS-PAGE separation step where uneven gel polymerization causes non-parallel band artifacts."
Bilibili (China-Accessible Mirrors) ★ 70
Western blot hands-on: Protein extraction through detection
"Step-by-step hands-on protocol with emphasis on troubleshooting common pitfalls, useful for avoiding casting/polymerization errors."
Source: sigmaaldrich.com ↗
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