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Western Blot (Sigma Protocol) minor

Non-parallel Bands

Symptom
Protein bands appear curved, wavy, or not parallel to each other
Common Causes
  1. 1 Gel has not polymerized evenly or parallel
Solutions
  1. 1 Review gel preparation procedure to ensure even polymerization
  2. 2 Ensure casting apparatus is level during gel polymerization
Related Video (3)
Cell Signaling Technology ★ 92
Western Blot Troubleshooting Guide
"Dedicated troubleshooting guide for Western blotting that directly addresses diagnostic problems and solutions, most likely to cover gel polymerization issues."
Bilibili (China-Accessible Mirrors) ★ 78
Western blot hands-on: Protein extraction through detection
"Hands-on protocol with explicit emphasis on troubleshooting common pitfalls, including gel preparation and SDS-PAGE execution where polymerization evenness is critical."
Bio-Rad Laboratories ★ 71
Western Blotting
"Demonstrates proper SDS-PAGE gel separation technique using established Bio-Rad protocol, showing correct gel polymerization and band formation."
Source: sigmaaldrich.com ↗
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