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Lipid Transfection minor

Granular Precipitate on Cells

Symptom
Small granular precipitate visible microscopically on cells after adding transfection complexes
Common Causes
  1. 1 Excess EDTA present in DNA dilution buffer
  2. 2 Excess cationic lipid present during complex formation
  3. 3 EDTA concentration >0.3 mM in TE buffer
Solutions
  1. 1 Dilute DNA in water instead of TE buffer
  2. 2 If TE preferred, use EDTA concentrations <0.3 mM
  3. 3 Ensure cationic lipid concentrations do not exceed recommended amounts
  4. 4 Note: precipitate presence/absence is not indicative of transfection performance
Related Video (2)
Bilibili (China-Accessible Mirrors) ★ 78
Cell Transfection Operation: Common Mistakes Avoided
"Hands-on protocol with troubleshooting and common pitfalls, useful for avoiding fixation and precipitate-forming mistakes like excess EDTA."
YouTube (Curated Tutorials) ★ 75
Plasmid DNA Transfection Protocol
"Optimized Lipofectamine LTX/Plus protocol video directly matches the lipid transfection technique and shows proper DNA/reagent handling to prevent precipitation."
Source: thermofisher.com ↗
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