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Lipid Transfection minor

Granular Precipitate on Cells

Symptom
Small granular precipitate visible microscopically on cells after adding transfection complexes
Common Causes
  1. 1 Excess EDTA present in DNA dilution buffer
  2. 2 Excess cationic lipid present during complex formation
  3. 3 EDTA concentration >0.3 mM in TE buffer
Solutions
  1. 1 Dilute DNA in water instead of TE buffer
  2. 2 If TE preferred, use EDTA concentrations <0.3 mM
  3. 3 Ensure cationic lipid concentrations do not exceed recommended amounts
  4. 4 Note: precipitate presence/absence is not indicative of transfection performance
Related Video (2)
Bilibili (China-Accessible Mirrors) ★ 85
Cell Transfection Operation: Common Mistakes Avoided
"Directly addresses common transfection mistakes and troubleshooting, including precipitate-related issues."
Bilibili (China-Accessible Mirrors) ★ 70
Cell Transfection Principles and Complete Protocol
"Includes optimization tips relevant to avoiding reagent-related artifacts like granular precipitates."
Source: thermofisher.com ↗
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