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Lipid Transfection severe

Cell Death After RNAiMAX Transfection

Symptom
Significant cell death observed following transfection with Lipofectamine RNAiMAX reagent
Common Causes
  1. 1 Antibiotics added to medium during transfection
  2. 2 Serum-free media incompatible with Lipofectamine RNAiMAX
Solutions
  1. 1 Do not add antibiotics to medium during transfection
  2. 2 Test serum-free media for compatibility with Lipofectamine RNAiMAX reagent before use
Related Video (2)
YouTube (Curated Tutorials) ★ 90
siRNA Transfection Protocol
"Directly demonstrates the Lipofectamine RNAiMAX siRNA transfection protocol implicated in this failure."
Bilibili (China-Accessible Mirrors) ★ 70
Cell Transfection Operation: Common Mistakes Avoided
"Highlights transfection troubleshooting and common pitfalls, useful for avoiding the antibiotic-related cell death issue."
Source: thermofisher.com ↗
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