IP Immunoprecipitation Experiment Protocol Steps

severe

Bead-Antibody Isotype Incompatibility

No or very weak target protein signal despite using adequate antibody concentration, suggesting inefficient antibody capture by the beads due to isotype incompatibility.

💡 5 · ✓ 5

moderate

Extra Bands at 50 kDa and 25 kDa Masking Target

Western blot shows strong bands at approximately 50 kDa (heavy chain) and 25 kDa (light chain) that may mask or interfere with the target protein band of interest.

💡 3 · ✓ 3