ELISA Experimental Protocol Demonstration

severe

Non-specific Background Signal Too High in Competitive ELISA

High background optical density is observed across wells, reducing signal-to-noise ratio and making it difficult to distinguish specific binding from non-specific interactions.

💡 4 · ✓ 4

severe

Serial Dilution Calculation or Execution Error

Standard curve shows irregular pattern or non-monotonic response, with individual points deviating significantly from expected sigmoidal or linear trend.

💡 5 · ✓ 6