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Cell Biology Bilibili (China-Accessible Mirrors)

Cell Transfection Principles and Complete Protocol

🚨 Failure Case Library (4) + Submit your own case

severe
Very few cells are fluorescent — transfection efficiency is extremely low
Microscopy shows scattered positive cells; flow cytometry confirms positivity rate is very low (< 5 %).
💡 5 · ✓ 5
severe
Cytotoxicity After Transfection
Reduced cell viability and cell death observed following transfection procedure
💡 7 · ✓ 7
moderate
Lipid Reagent Accidentally Frozen
Transfection reagent was frozen instead of stored at 4°C, potentially affecting performance
💡 2 · ✓ 3
moderate
Transfections Not Reproducible
Transfection efficiency shows high variability between experiments and among replicates
💡 5 · ✓ 7
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