LNA Technology for NGS and PCR Optimization

severe

Probe Integrity Compromised During Storage or Synthesis

Target RNA depletion efficiency is significantly reduced or absent. Sequencing shows minimal reduction in targeted reads despite proper protocol execution.

💡 3 · ✓ 4

severe

Improper Temperature Ramp During Probe Hybridization

Probe hybridization step was unsuccessful resulting in poor depletion. Target RNA removal is inconsistent or incomplete across replicate samples.

💡 4 · ✓ 5

moderate

Non-Uniform Depletion Across Targeted Sequences

Depletion efficiency varies significantly across different targeted sequences. Some target regions show good depletion while others remain at high levels.

💡 4 · ✓ 5