Plasmid DNA Extraction Miniprep Protocol

severe

Low Plasmid Yield Due to Incomplete Lysis

Plasmid miniprep yields are significantly below expected levels. A260/280 ratio may be normal but total DNA concentration is low.

💡 5 · ✓ 5

severe

Mini-prep yields almost no plasmid DNA

Eluate DNA concentration is very low; diagnostic digest and gel show no detectable target band.

💡 4 · ✓ 4

severe

Incomplete Restriction Enzyme Digestion

Partial digestion observed with both expected fragments and uncut substrate visible on gel; incomplete linearization of plasmid DNA.

💡 6 · ✓ 6

severe

Low Plasmid Quality Due to Degradation or Denaturation

Plasmid DNA shows smearing on gel, poor A260/280 ratio, or fails in downstream applications despite adequate concentration. Multiple bands or reduced supercoiled form may be visible.

💡 5 · ✓ 5