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Microbiology Bilibili (China-Accessible Mirrors)

E. coli Competent Cell Preparation, Plasmid Transformation, and DNA Extraction

🚨 Failure Case Library (2) + Submit your own case

critical
No DNA Recovered from Plasmid Miniprep
No detectable plasmid DNA is recovered after miniprep purification. Column elution yields no nucleic acid by spectrophotometry or gel electrophoresis.
💡 4 · ✓ 4
severe
Low Plasmid Yield Due to Incomplete Lysis
Plasmid miniprep yields are significantly below expected levels. A260/280 ratio may be normal but total DNA concentration is low.
💡 5 · ✓ 5
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