Cell Passaging Steps and Precautions

severe

Contamination from Aerosol Generation

Multiple cultures becoming contaminated simultaneously or in sequence. Contamination spreading despite apparently good technique. Cross-contamination between neighboring cultures.

💡 7 · ✓ 9

moderate

Incubator-Associated Mold Contamination

Recurring fungal contamination in cultures after incubation. Multiple cultures affected over time. Visible mold growth may appear on incubator surfaces. Persistent contamination despite replacing affected cultures.

💡 6 · ✓ 8