Cell Passaging Steps and Precautions

severe

Bacterial Contamination with Rapid Turbidity

Rapid-onset turbidity and color change of culture medium (when phenol red is present). Medium becomes cloudy and pH indicator changes color quickly.

💡 7 · ✓ 9

severe

Contamination from Aerosol Generation

Multiple cultures becoming contaminated simultaneously or in sequence. Contamination spreading despite apparently good technique. Cross-contamination between neighboring cultures.

💡 7 · ✓ 9