Home › Molecular Biology › Hands-on plasmid construction guide
Molecular Biology Bilibili (China-Accessible Mirrors)

Hands-on plasmid construction guide

🚨 Failure Case Library (2) + Submit your own case

critical
Confirmed colony later shows recombination or deletion
Initial screening looked correct, but on re-verification the insert is missing pieces or the vector has rearranged.
💡 4 · ✓ 4
severe
Double digest band pattern does not match prediction
Picked colonies grow normally but the diagnostic double-digest yields bands of unexpected size on the gel.
💡 5 · ✓ 5
💬 Comments coming soon