Hands-on plasmid construction guide

severe

Double digest band pattern does not match prediction

Picked colonies grow normally but the diagnostic double-digest yields bands of unexpected size on the gel.

💡 5 · ✓ 5

moderate

Star Activity Generating Extra Bands

Extra bands appear on gel beyond expected digestion products. Enzyme cleaves at non-canonical sites with relaxed sequence specificity under suboptimal conditions.

💡 5 · ✓ 5