Bio-Rad qPCR Instrument Operation Protocol

severe

Poor Ct reproducibility — high within-group variability

Technical replicates of the same sample have SD > 0.5 cycles; standard curve linearity is poor; loading order influences results.

💡 5 · ✓ 5

severe

PCR ReadyMix Works for PCR but Fails in qPCR

ReadyMix produces amplification in standard PCR but completely fails in real-time qPCR applications; equivalent products from other suppliers work well

💡 3 · ✓ 3

moderate

Amplification Plots Dip Below Zero Due to Incorrect Baseline Settings

Amplification plots are clearly abnormal with sections dipping below zero dR; data cannot be used as presented; plots appear distorted

💡 3 · ✓ 4