PCR practical operation video demonstration

critical

No Band Due to Incorrect Component Concentrations

No visible band or very faint band on gel despite proper thermal cycling parameters. The reaction components may be at suboptimal or inhibitory concentrations.

💡 5 · ✓ 5

severe

No Band or Faint Band Due to Thermal Cycling Errors

No visible band or very faint band appears on the gel after PCR amplification. This indicates insufficient or failed amplification of the target sequence.

💡 6 · ✓ 6

moderate

PCR污染和携带污染

Unexpected products appear in negative controls or multiple samples show identical non-specific bands, indicating contamination from previous amplifications or environmental sources.

💡 4 · ✓ 7

moderate

Nonspecific Bands or Primer-Dimers Due to Thermal Cycle Issues

Gel shows multiple bands instead of single target band, or primer-dimers appear as small molecular weight products. The desired product may be present but accompanied by unwanted amplification artifacts.

💡 6 · ✓ 6

moderate

JumpStart Taq ReadyMix Underperforming Due to Incorrect Hot Start Protocol

JumpStart Taq ReadyMix does not work as well as similar products from different suppliers; amplification is weak or absent despite correct assay design

💡 3 · ✓ 3