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Immunology Bilibili (China-Accessible Mirrors)

IHC Immunohistochemistry Practical Operation Demo

🚨 Failure Case Library (2) + Submit your own case

severe
Over-Amplified Signal with Biotin-Based Detection Systems
Excessive background staining when using amplification techniques or biotin-based detection, either from high signal amplification or endogenous biotin binding to streptavidin/avidin.
💡 4 · ✓ 4
moderate
Spotty or Uneven Background Staining Pattern
Staining pattern shows irregular, patchy, or spotty background with uneven distribution across tissue sections, indicating technical artifacts rather than biological variation.
💡 3 · ✓ 3
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