Paraffin Section IHC Experimental Workflow

severe

Tissue detachment / sections fall off the slide

Tissue falls off during staining; sections become broken or missing; results cannot be interpreted.

💡 4 · ✓ 4

moderate

FFPE tissue shows no staining from incomplete deparaffinization

Paraffin-embedded tissue sections show no immunostaining. Residual paraffin creates a physical barrier preventing antibody access to tissue antigens.

💡 3 · ✓ 4