Choose the Gemini XPS as the plate reader machine in Softmax Pro 6. This is the first step to initialize the instrument setup.
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2
Configure wavelength and plate settings
Access Settings and Kinetic Mode, then set the excitation wavelength to 544 nm and emission wavelength to 590 nm. Select 96-well Standard Opaque plate type.
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3
Set grid area and timing parameters
Select the appropriate number of rows based on your sample count and configure the run time to 1 hour with 30-second intervals. Set the shake option to 5 seconds before the first read.
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4
Set up the standard curve
Open Template Editor and create standard curve points in duplicate, naming each sample and entering its corresponding concentration. This establishes the reference data for analysis.
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5
Name and configure experimental samples
Add and name all test samples according to your study design, specifying the dilution used for each sample. The naming will auto-increment for subsequent samples.
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6
Finalize setup and begin reading
Click OK to save all template settings, then place your prepared plate into the machine and click the Read button to start the measurement process.