Home › Biochemistry › How to do your protein assay in less than 30 minutes - Innovation Insights series
Steps
  1. 1 Identify the protein assay challenge --:--
  2. 2 Choose the Qubit broad range assay 00:16
  3. 3 Use a two-point standard curve 00:26
  4. 4 Add samples directly without dilution 00:34
  5. 5 Measure with the Qubit 4 fluorometer 00:50
Biochemistry Thermo Fisher Scientific

How to do your protein assay in less than 30 minutes - Innovation Insights series

Protocol
Difficulty
intermediate

Steps

1
Identify the protein assay challenge

The video opens by asking whether your protein assay takes more than 30 minutes, framing the need for a faster method.

▶ --:--
2
Choose the Qubit broad range assay

Introduce the Invitrogen Qubit protein broad range assay as the solution for determining concentrations of purified proteins, lysates, serum, and complex mixtures.

▶ 00:16
3
Use a two-point standard curve

Describe the room-temperature assay format with only a two-point standard curve, simplifying setup and reducing hands-on time.

▶ 00:26
4
Add samples directly without dilution

Highlight the broad dynamic range that allows most samples to be added directly to the assay, eliminating dilutions and further saving time.

▶ 00:34
5
Measure with the Qubit 4 fluorometer

Combine the Invitrogen Qubit protein broad range assay with the Invitrogen Qubit 4 fluorometer to accurately measure protein as well as DNA and RNA.

▶ 00:50
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