Home › Immunology › A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
Immunology JoVE (Open Access) Citable · DOI

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

DOI: 10.3791/53485-v
What you'll learn
  • ✓ Prepare antibody cocktails semi-automatically
  • ✓ Minimize reagent dispensing variability
  • ✓ Perform immunophenotypic analysis of blood
  • ✓ Ensure reagent stability and reproducibility
Protocol

Biopharma Insights Whole blood Immunophenotyping is indispensable for monitoring the human immune system. However, the number of reagents required and the instability of specific fluorochromes in premixed cocktails necessitates daily reagent preparation. Here we show that semi-automated preparation of staining antibody cocktail helps establish reliable immunophenotyping by minimizing variability in reagent dispensing.

Difficulty
intermediate
Total time
~1-2 hrs per sample set
Biosafety
BSL-2

Steps

1
Operate semi-automated cocktail preparation

Use the semi-automated system to dispense antibodies for staining cocktails.

▶ 00:58
2
Assess variability in immunophenotyping results

Evaluate the low variability in whole blood immunophenotyping achieved with semi-automated cocktail preparation.

▶ 06:25
3
Conclude with protocol recommendations

Review the advantages of semi-automated preparation for reliable immunophenotyping.

▶ 07:04
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