Biopharma Insights A protocol for comprehensive extraction of lipids, metabolites and proteins from biological tissues using one sample is presented.
Mechanically disrupt the tissue using grinding to prepare for extraction.
Divide the disrupted tissue into aliquots for subsequent extraction steps.
Add solvents to induce phase separation, fractionating metabolites, lipids, and proteins.
Collect and aliquote the polar and hydrophobic layers for further analysis.
Concentrate the fractions and analyze the lipid content using UPLC-MS.
Analyze the polar and semi-polar metabolite fractions using UPLC-MS.
Observe example chromatograms of lipids, metabolites, and proteins from Arabidopsis leaf extracts.
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