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Cell Biology JoVE (Open Access) Citable · DOI

Absolute Quantum Yield Measurement of Powder Samples

DOI: 10.3791/3066-v
What you'll learn
  • ✓ Set up and calibrate an integrating sphere system for quantum yield measurement
  • ✓ Measure absolute quantum yield of powder samples using fluorescence spectroscopy
  • ✓ Calculate chromaticity coordinates from spectral data
  • ✓ Interpret and report quantum yield results with proper corrections
Protocol

In this video we will demonstrate measuring and calculating absolute quantum yield and chromaticity coordinates directly in powder samples using the Hitachi F-7000 Quantum Yield Measuring System.

Difficulty
advanced
Total time
~2–3 hours per sample (including calibration and calculations)

Steps

1
Set up integrating sphere and acquire reference factors

Initialize the Hitachi F-7000 Quantum Yield Measuring System and acquire baseline integration sphere factors required for subsequent measurements.

▶ 01:48
2
Acquire diffuser data and correction factors

Measure diffuser reference data and calculate integrating sphere correction factors to normalize for system geometry and optical properties.

▶ 02:47
3
Measure sodium salicylate powder sample

Acquire fluorescence and excitation data for the sodium salicylate reference powder using the calibrated integrating sphere system.

▶ 04:51
4
Calculate absolute quantum yield value

Apply correction factors and integrate spectral data to compute absolute quantum yield as a percentage for the measured sample.

▶ 08:28
5
Calculate chromaticity coordinates from spectra

Derive CIE chromaticity coordinates (x, y) from the measured emission spectrum using standard color-matching functions.

▶ 11:46
6
Report quantum yield and chromaticity results

Present final quantum yield percentage and chromaticity coordinates for the sodium salicylate powder sample.

▶ 13:10
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