Cell Biology JoVE (Open Access) Citable · DOI

Automated 90Sr Separation and Preconcentration in a Lab-on-Valve System at Ppq Level

Name: Automated 90Sr Separation and Preconcentration in a Lab-on-Valve System at Ppq Level
Uploaded: 2026-07-01
Duration: 8 min 53 s
Description: A quick, automated and portable system for the separation and determination of radiostrontium in aqueous samples, using Sr-resin and multi sequential flow ...

DOI: 10.3791/57722-v

What you'll learn

✓ Operate a portable autoRAD lab-on-valve system for radiometric analysis
✓ Perform automated 90Sr separation and preconcentration from aqueous samples
✓ Interpret radiometric detection results at ppq-level sensitivity
✓ Execute system cleaning and resin loading protocols for radioactive assays

Protocol

Here, we present the portable autoRAD platform for quick radiometric separation and determination of 90Sr, an important fission product highly pertinent to nuclear waste.

Difficulty

advanced

Total time

~3–4 hours per sample batch (including system prep, loading, and detection)

Biosafety

BSL-2

Steps

Prepare and clean lab-on-valve system

Execute system preparation and cleaning procedures to prepare the portable autoRAD platform for radiometric analysis. This includes fluidic line flushing and component decontamination.

▶ 00:30

Load resin and detect 90Sr activity

Load separation resin into the lab-on-valve system and configure detection parameters for 90Sr radiometric measurement at ppq sensitivity levels.

▶ 03:28

Analyze 90Sr activity in aqueous samples

Measure and quantify 90Sr activity concentrations in aqueous test samples using the automated separation and preconcentration workflow.

▶ 07:13

💬 Comments coming soon