Home›Immunology›Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma
ImmunologyJoVE (Open Access)Citable · DOI
Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma
DOI: 10.3791/53287-v
What you'll learn
✓Establish luciferase-expressing GL261 glioma cells for bioluminescent tracking
✓Perform intracranial tumor cell implantation in immunocompetent mice
✓Monitor in vivo glioblastoma growth using bioluminescence imaging
✓Apply protocol to immunotherapy and tumor immunology studies
Protocol
GL261 glioma cells provide a useful immunocompetent animal model of glioblastoma. The goals of this protocol are to demonstrate proper techniques for monitoring intracranial tumor growth using in vivo bioluminescence imaging, and to verify the utility of luciferase-modified GL261 cells for studying tumor immunology and immunotherapeutic approaches for treating glioblastoma.
Transduce GL261 glioma cells with firefly luciferase construct to enable bioluminescent signal detection. Verify stable expression in cultured cells.
▶ 01:00
2
Assess in vitro luciferase-expressing cell proliferation
Quantify proliferation kinetics of luciferase-modified GL261 cells in culture using a luminescence-based assay to confirm viability.
▶ 02:31
3
Implant tumor cells intracranially in mice
Surgically inject luciferase-expressing GL261 cells into the brains of anesthetized immunocompetent mice via stereotactic apparatus.
▶ 03:40
4
Image tumor growth via bioluminescence detection
Perform serial in vivo bioluminescence imaging of tumor-bearing mice following luciferin substrate administration to track intracranial glioblastoma progression.