Contextual and Cued Fear Conditioning Test Using a Video Analyzing System in Mice

Understand the overall experimental workflow and safety considerations for conducting fear conditioning tests in mice.

Set up the conditioning chamber with appropriate lighting, flooring, and chamber context to establish baseline conditions for fear learning.

Modify the chamber environment and settings to deliver auditory cues distinct from the conditioning context during the cued test phase.

Present paired tone and foot-shock stimuli to establish fear memory in mice, following the protocol timeline for stimulus delivery.

Reintroduce the mouse to the original conditioning chamber without stimuli and measure freezing behavior as an indicator of contextual fear memory.

Present the conditioned tone cue in a modified chamber environment and quantify freezing to assess cue-specific fear memory.

Review representative results from video analysis showing freezing behavior quantification across conditioning, context, and cued test phases.