Immunology JoVE (Open Access) Citable · DOI

Depletion of Specific Cell Populations by Complement Depletion

Name: Depletion of Specific Cell Populations by Complement Depletion
Uploaded: 2026-08-05
Duration: 6 min 17 s
Description: The purification of immune cell populations is often required in order to study their unique functions. In particular, molecular approaches such as ...

DOI: 10.3791/1487-v

What you'll learn

✓ Select appropriate complement and antibodies
✓ Prepare rabbit complement for depletion
✓ Perform complement-mediated cell depletion
✓ Assess purity of depleted cell populations

Protocol

Biopharma Insights To effectively study the function of immune cell populations their purification is often required. Complement depletion is a fast and inexpensive technique for the isolation of immune cell populations with high purity.

Difficulty

intermediate

Total time

~2-3 hours per sample

Biosafety

BSL-1

Steps

Prepare rabbit complement and antibodies

Thaw or reconstitute rabbit complement and select the specific antibody targeting the cell population to deplete.

▶ 01:18

Perform complement depletion of target cells

Incubate cells with the selected antibody and rabbit complement to lyse the targeted cell population.

▶ 02:22

Analyze results of complement depletion

Evaluate the efficiency of depletion by assessing the remaining cell populations.

▶ 05:06

💬 Comments coming soon