Home › Microbiology › Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Microbiology JoVE (Open Access) Citable · DOI

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

DOI: 10.3791/56234-v
What you'll learn
  • ✓ Design microbial cultivation media iteratively
  • ✓ Operate automated microbioreactor for cultivations
  • ✓ Use Kriging-based experimental design workflow
  • ✓ Apply lab robotics for media preparation
Protocol

Biopharma Insights This manuscript describes a generic approach for tailor-made design of microbial cultivation media. This is enabled by an iterative workflow combining Kriging-based experimental design and microbioreactor technology for sufficient cultivation throughput, which is supported by lab robotics to increase reliability and speed in liquid handling media preparation.

Difficulty
advanced
Total time
~2-3 days per optimization cycle
Biosafety
BSL-1

Steps

1
Prepare seed culture and automated media

Grow a seed culture and use lab robotics to prepare tailored media formulations for the main culture.

▶ 01:05
2
Quantify product and preprocess raw data

Measure product yields from the microbioreactor runs and preprocess the raw data for subsequent statistical analysis.

▶ 02:53
3
Evaluate results iteratively with Kriging

Apply the iterative workflow including Kriging-based experimental design to update media compositions and perform final statistical evaluation.

▶ 04:22
4
Conclude with optimized media formulation

Summarize the outcome and confirm the tailored media formulation that improves heterologous protein production.

▶ 05:31
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