Home › Analytical Chem › Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation
Analytical Chem JoVE (Open Access) Citable · DOI

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

DOI: 10.3791/57434-v
What you'll learn
  • ✓ Perform solid phase extraction for water samples
  • ✓ Set up UV irradiation for pharmaceutical degradation
  • ✓ Identify pharmaceuticals using HPLC-ESI-Q-TOF-MS
  • ✓ Model degradation kinetics from MS data
Protocol

Biopharma Insights We present a protocol for non-targeted analysis using time of flight mass spectrometry as a perfect tool to identify pharmaceuticals in waters. We demonstrate the application of UV irradiation for their elimination. Analysis involving irradiation, compound isolation, identification and kinetic modelling of the degradation profiles is illustrated.

Difficulty
advanced
Total time
~1-2 days per sample set
Biosafety
BSL-1

Steps

1
Extract pharmaceuticals via solid phase extraction

Concentrate water samples using solid phase extraction to isolate pharmaceuticals for analysis.

▶ 00:39
2
Perform UV irradiation and LC-MS analysis

Irradiate samples with UV light and analyze both non-targeted and targeted compounds using LC-MS and MS/MS.

▶ 01:53
3
Identify pharmaceuticals in river water

Use high-resolution mass spectrometry data to identify pharmaceuticals present in river water samples.

▶ 03:29
4
Evaluate degradation kinetics and conclude

Model the degradation profiles and summarize the effectiveness of UV treatment for pharmaceutical removal.

▶ 04:53
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