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Immunology JoVE (Open Access) Citable · DOI

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Name: Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
Uploaded: 2026-10-23
Duration: 7 min 19 s
Description: Learn to validate human microRNA targets using the LightSwitch luciferase assay system with 3'UTR-reporter constructs and miRNA mimics in adherent cells. This functional assay enables researchers to confirm predicted miRNA-UTR interactions experimentally.

DOI: 10.3791/3343-v

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