Microbiology JoVE (Open Access) Citable · DOI

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

Name: Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
Uploaded: 2026-08-05
Duration: 12 min 40 s
Description: Mesenchymal stem/stromal cells (MSC) are promising candidates for use in cell-based therapies. In most cases, therapeutic response appears to be cell-dose ...

DOI: 10.3791/54204-v

What you'll learn

✓ Dissect fetal and maternal placental tissues
✓ Isolate mononuclear cells from digested tissue
✓ Culture and expand placental mesenchymal stem/stromal cells
✓ Assay for MSC characteristics

Protocol

Biopharma Insights Herein we describe methods for the dissection of fetal and maternal tissues from human term placenta, followed by isolation and expansion of mesenchymal stem/stromal cells (MSC) from these tissues.

Difficulty

intermediate

Total time

~6-8 hours per sample

Model organism

Human placenta tissue

Biosafety

BSL-2

Steps

Prepare placenta and identify anatomy

Prepare the human term placenta and identify the fetal and maternal tissue regions for dissection.

▶ 03:53

Dissect decidua tissue

Dissect the maternal decidua (D) tissue from the placenta.

▶ 04:40

Dissect chorionic plate and villi

Dissect the chorionic plate (CP) and chorionic villi (CV) tissues from the placenta.

▶ 05:40

Mince and digest placental tissue

Mince the placental tissue into small pieces and perform enzymatic digestion.

▶ 06:36

Collect mononuclear cells and plate

Collect mononuclear cells from the digested tissue and plate them into culture flasks.

▶ 07:25

Expand isolated MSCs

Culture the isolated mesenchymal stem/stromal cells and expand them in vitro.

▶ 09:33

Assay for MSC characteristics

Perform representative assays to confirm MSC characteristics, such as differentiation potential and surface markers.

▶ 09:57

💬 Comments coming soon