Home › Microbiology › Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
Microbiology JoVE (Open Access) Citable · DOI

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

DOI: 10.3791/2950-v
What you'll learn
  • ✓ Prepare plant samples rapidly using microwaves
  • ✓ Perform negative staining for TEM
  • ✓ Diagnose plant virus diseases by TEM
  • ✓ Interpret TEM images of virus particles
Protocol

Biopharma Insights This study describes a method that allows the rapid and clear diagnosis of plant virus diseases in about half a day by using a combination of microwave assisted plant sample preparation for transmission electron microscopy and negative staining methods.

Difficulty
intermediate
Total time
~0.5 day
Biosafety
BSL-1

Steps

1
Microwave assist sample preparation

Use microwave irradiation to rapidly fix and process plant tissue for electron microscopy.

▶ 01:38
2
Trim and section embedded samples

Trim the resin-embedded sample and cut ultrathin sections for TEM.

▶ 04:19
3
Apply negative staining to sections

Stain the ultrathin sections with a negative stain to enhance contrast of virus particles.

▶ 05:36
4
Acquire and analyze TEM images

View stained sections under transmission electron microscope and capture images for diagnosis.

▶ 06:41
5
Assess representative TEM results

Evaluate the obtained TEM images to identify viral particles and confirm disease diagnosis.

▶ 07:52
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