Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

critical

No Spots or Very Weak Signal

Few or no spots are visible after development, or spots are extremely faint and difficult to detect even though cytokine-secreting cells should be present.

💡 5 · ✓ 5

severe

Physical Membrane Damage and Artifacts

Membrane shows physical damage, scratches, or irregular patterns. Spots may appear distorted or artifacts present as non-specific signals.

💡 4 · ✓ 4

moderate

Over-Developed Plate with Excessive Background Color

The entire membrane has excessive background color development, reducing contrast between spots and background. Spots may be obscured by high background signal.

💡 4 · ✓ 4