Biochemistry JoVE (Open Access) Citable · DOI

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Name: Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants
Uploaded: 2026-08-05
Duration: 8 min 48 s
Description: The capacity to introduce exogenous proteins and express (or down-regulate) specific genes in plants provides a powerful tool for fundamental research as ...

DOI: 10.3791/54972-v

What you'll learn

✓ Prepare peptide-based formulations for plant transfection
✓ Infiltrate plant leaves with peptide-DNA complexes
✓ Assess transfection efficiency via reporter expression
✓ Distinguish peptide carriers for gene/protein delivery

Protocol

Biopharma Insights Existing methods for the modification of plants have limited applicability. The novel peptide-derived technology proposed here promises both simplicity and efficiency in the introduction of exogenous protein or genes into the desired intracellular compartments of intact plants.

Difficulty

intermediate

Total time

~2-3 hours per batch

Model organism

Nicotiana benthamiana

Biosafety

BSL-1

Steps

Characterize peptide-based formulations

Prepare and analyze peptide formulations to ensure proper complex formation for delivery.

▶ 01:02

Infiltrate plant leaves with formulations

Infuse the peptide-based complexes into intact plant leaves using a needleless syringe.

▶ 02:28

Evaluate transfection efficiency

Assess the uptake and expression of delivered genes or proteins in the infiltrated plant tissues.

▶ 03:28

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