Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

severe

DNA Smearing on Agarose Gel After Digestion

Restriction digest product appears as a smear rather than discrete bands on agarose gel, indicating DNA degradation or enzyme-DNA complex formation.

💡 3 · ✓ 4

moderate

DNA Smear on Agarose Gel

Digested DNA appears as a smear rather than discrete bands on agarose gel. No sharp bands are visible across a wide molecular weight range.

💡 4 · ✓ 4

minor

Enzyme-DNA Complex Causing Larger Bands

Larger molecular weight bands than expected appear on gel. Restriction enzyme remains bound to cleaved DNA substrate, retarding migration.

💡 3 · ✓ 3