Home › Analytical Chem › Probiotic Studies in Neonatal Mice Using Gavage
Analytical Chem JoVE (Open Access) Citable · DOI

Probiotic Studies in Neonatal Mice Using Gavage

DOI: 10.3791/59074-v
What you'll learn
  • ✓ Perform intra-esophageal gavage in neonatal mice
  • ✓ Collect intestinal samples for colonization analysis
  • ✓ Compare daily versus every-other-day probiotic dosing
  • ✓ Assess probiotic transfer via co-housing
Protocol

Biopharma Insights This study details the process of gavaging precise amounts of probiotics to neonatal mice. The experimental set-up was optimized to include but is not limited to probiotic dosing, methods of administration, and quantification of bacteria in the intestines.

Difficulty
intermediate
Total time
~2-3 hours for gavage and sample collection
Model organism
Neonatal mouse
Biosafety
BSL-1

Steps

1
Perform intra-esophageal gavage on neonatal mice

Administer precise probiotic doses to neonatal mice via intra-esophageal gavage on day of life 2.

▶ 00:04
2
Collect intestinal samples for colonization analysis

Harvest intestinal tissue from gavaged mice to quantify bacterial colonization.

▶ 05:19
3
Compare daily versus every-other-day probiotic dosing

Evaluate Lactobacillus plantarum colonization with gavage administered daily or every other day.

▶ 08:38
4
Assess probiotic transfer via co-housing

Co-house untreated mice with gavaged mice to test probiotic transmission between individuals.

▶ 09:03
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