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Organic Chemistry JoVE (Open Access) Citable · DOI

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

DOI: 10.3791/57297-v
What you'll learn
  • ✓ Synthesize gold nanoparticles in situ on nanoprobe surfaces for signal enhancement
  • ✓ Apply chloroauric acid reduction protocol to diverse nanoparticle types
  • ✓ Optimize vertical flow paper-based biosensing assays using nanoprobe enhancement
Protocol

In this work, a protocol for signal enhancement of nanoprobe-based biosensing is presented. The protocol is based on the reduction of chloroauric acid onto the surface of existing nanoprobes that consist of gold, silver, silica or iron-oxide nanoparticles.

Difficulty
intermediate
Total time
~2–4 hours per assay batch
Biosafety
BSL-1

Steps

1
Functionalize gold nanoparticles with targeting ligands

Prepare and functionalize gold nanoparticles (AuNPs) with surface coatings or ligands such as antibodies or aptamers to create capture-ready nanoprobes for biosensing applications.

▶ 00:33
2
Enhance signal in vertical flow paper assays

Apply the in situ gold nanoparticle synthesis protocol to vertical flow paper-based assay platforms, depositing chloroauric acid onto existing nanoprobes to amplify detection signals.

▶ 03:32
3
Quantify signal enhancement across nanoparticle types

Measure and compare signal enhancement results for IgG-modified gold, silver, silica, and iron-oxide nanoparticles to validate protocol effectiveness across diverse nanoprobe compositions.

▶ 05:50
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