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Cell Biology JoVE (Open Access) Citable · DOI

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

DOI: 10.3791/60316-v
What you'll learn
  • ✓ Establish orthotopic breast cancer model in mice
  • ✓ Measure tumor growth using calipers
  • ✓ Detect cancer cells via GFP and bioluminescence
  • ✓ Identify metastatic cells in lung tissue
Protocol

Biopharma Insights This work presets an advanced protocol to accurately assess tumor loading by detection of green fluorescent protein and bioluminescence signals as well as the integration of quantitative molecular detection technique.

Difficulty
advanced
Total time
~4-6 weeks for tumor development and analysis
Model organism
Mouse
Biosafety
BSL-2

Steps

1
Induce orthotopic breast cancer model

Inject cancer cells into the mammary fat pad to establish orthotopic tumors.

▶ 00:55
2
Perform in vivo bioluminescence and ex vivo fluorescence imaging

Use bioluminescence and GFP fluorescence to visualize primary tumors and metastases.

▶ 02:05
3
Detect metastatic breast cancer cells molecularly

Apply quantitative molecular techniques to identify metastatic cells in biological samples.

▶ 04:19
4
Detect lung metastatic breast cancer cells

Specifically analyze lung tissue for the presence of metastatic breast cancer cells.

▶ 06:27
5
Analyze representative BLI and GFP results

Review representative images and signals to confirm tumor burden and metastatic spread.

▶ 07:29
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