Home › Botany › Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction
Botany JoVE (Open Access) Citable · DOI

Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction

DOI: 10.3791/4376-v
What you'll learn
  • ✓ Perform donor liver harvest in rats
  • ✓ Prepare 50% partial liver graft ex vivo
  • ✓ Execute orthotopic transplantation with arterial reconstruction
  • ✓ Assess graft survival and hepatocellular damage
Protocol

Biopharma Insights Orthotopic liver transplantation in rats is an indispensable experimental model for biomedical research. Here we present our surgical procedures for orthotopic rat liver transplantation with hepatic arterial reconstruction using a 50% partial graft.

Difficulty
advanced
Total time
~4-6 hrs/surgery
Model organism
Rat
Biosafety
BSL-1

Steps

1
Harvest donor liver with vasculature

Dissect and perfuse the donor rat liver, isolating the vasculature and biliary tree for graft procurement.

▶ 01:07
2
Prepare partial graft ex vivo

Reduce the donor liver to a 50% partial graft and prepare cuff anastomoses on the bench.

▶ 06:30
3
Implant graft in recipient rat

Perform recipient hepatectomy and orthotopically implant the partial graft with hepatic arterial reconstruction.

▶ 10:02
4
Evaluate survival and hepatocellular damage

Monitor recipient survival and assess hepatocellular damage in the implanted liver grafts.

▶ 17:46
5
Conclude surgical procedure

Summarize the key steps and considerations for successful partial orthotopic liver transplantation in rats.

▶ 18:24
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