PCR Tube Crushing or Deformation Under Lid Pressure

Symptom

PCR tubes become crushed, collapsed, or deformed after thermal cycling, potentially causing sample loss or compromised seal integrity. Tubes may show visible damage especially when using tube strips.

1. 1 Incompatible cap style or tube design not verified for specific thermal cycler model
2. 2 Excessive pressure from thermal cycler lid due to overtightening or malfunctioning spring mechanism
3. 3 Uneven distribution of tubes/strips across block creating pressure imbalance under lid

1. 1 Use PCR plastics selection tool to verify tube and cap style compatibility with thermal cycler manufacturer specifications
2. 2 Distribute tubes and strips evenly across block; place empty tubes if needed to balance lid pressure
3. 3 Verify thermal cycler lid is not overtightened and spring/closing mechanism functions correctly; consult manufacturer guides on tray and retainer usage

Related Video (2)

Addgene ★ 65

Polymerase Chain Reaction (PCR) Protocol

"Demonstrates the PCR process and tube use, but does not address crushing or cap/tube compatibility."

Bilibili (China-Accessible Mirrors) ★ 60

Step-by-Step PCR Experiment Protocol Guide

"Shows a basic PCR setup which may indirectly cover tube handling, but not specifically plastic deformation."