Signal Drift Across Plate (ELISA Development)

Question

Accepted Answer

Gradual change in signal intensity across the plate suggesting time-dependent variation during assay setup Common causes: 1) Interrupted assay setup causing time delays between wells 2) Reagents not at room temperature during pipetting Solutions: 1) Assay setup should be continuous - have all standards and samples prepared appropriately before commencing the assay 2) Ensure all reagents are at room temperature before pipetting into wells unless otherwise instructed in antibody inserts