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Molecular Biology Bilibili (China-Accessible Mirrors)

Expert hands-on: RNA extraction best practices

🚨 Failure Case Library (2) + Submit your own case

critical
Purified RNA Shows Degradation
Bioanalyzer or gel electrophoresis reveals smearing or loss of distinct RNA bands. RNA integrity number (RIN) is significantly reduced compared to input material.
💡 4 · ✓ 5
moderate
Low A260/230 Ratio Indicates Contaminant Carryover
Spectrophotometric analysis shows A260/230 ratio below 1.8-2.0, indicating residual guanidine salt or other chaotropic agent contamination in the purified RNA.
💡 4 · ✓ 5
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