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Immunocytochemistry (ICC) — Step-by-Step Protocol (Bio-Techne)

Name: Immunocytochemistry (ICC) — Step-by-Step Protocol (Bio-Techne)
Uploaded: 2026-06-06
Duration: 8 min 7 s
Description: Bio-Techne walkthrough of ICC: cell fixation, permeabilization, blocking, primary and fluorophore-conjugated secondary antibody staining, nuclear counterstain, mounting.

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critical

Reversed Fixation and Permeabilization Order

Poor cell morphology, increased autofluorescence, or loss of intracellular antigens when permeabilization is performed before fixation.

💡 4 · ✓ 4

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