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siRNA Transfection Experimental Protocol

🚨 Failure Case Library (2) + Submit your own case

severe
Cell Death After RNAiMAX Transfection
Significant cell death observed following transfection with Lipofectamine RNAiMAX reagent
💡 2 · ✓ 2
severe
siRNA: qPCR shows knockdown but Western blot shows no change
qPCR confirms target mRNA is significantly reduced, but the corresponding protein band on Western blot looks unchanged.
💡 5 · ✓ 4
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