Western Blot Complete Process First-Person View

moderate

Band Warping from Well Overloading

Individual lanes show warped or distorted bands while adjacent lanes appear normal. Affected lanes may show smearing, broadening, or lateral spreading of protein bands.

💡 4 · ✓ 4

moderate

Streaking and Uneven Migration from Buffer Issues

Bands show streaking, smearing, or uneven migration patterns. Multiple bands may merge or appear poorly resolved with inconsistent migration across the gel.

💡 5 · ✓ 5

moderate

Accidental Bead Loss During Aspiration

Inconsistent or absent target protein signal across replicate samples, with visible reduction in bead pellet volume, indicating beads were aspirated during wash or supernatant removal steps.

💡 3 · ✓ 4

minor

Uneven or Distorted Blot Pattern

Membrane shows irregular signal distribution with visible fingerprints, fold marks, or forceps imprints. Signal intensity varies across membrane surface in non-biological pattern.

💡 4 · ✓ 4