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Microscopy & Imaging Bilibili (China-Accessible Mirrors)

Immunofluorescence IF Protocol and Confocal Imaging

🚨 Failure Case Library (2) + Submit your own case

severe
True Signal Loss After Autofluorescence Quenching
Signal drops or disappears after applying Sudan Black B or other quenching treatments. Target staining reduced along with background. Tissue morphology may be affected.
💡 5 · ✓ 6
moderate
Inconsistent Antigen Retrieval Results
Antigen retrieval (AR) performance varies unpredictably across sections and targets. Harsh AR needed to recover signal suggests overfixation. Epitope accessibility inconsistent even with standardized protocols.
💡 5 · ✓ 6
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