Trizol RNA Extraction from Cells: Hands-On Protocol

critical

Purified RNA Shows Degradation

Bioanalyzer or gel electrophoresis reveals smearing or loss of distinct RNA bands. RNA integrity number (RIN) is significantly reduced compared to input material.

💡 4 · ✓ 5

severe

Low RNA Yield After Cleanup

Recovered RNA concentration is significantly lower than expected based on input amount. Spectrophotometric measurement shows inadequate RNA quantity for downstream applications.

💡 5 · ✓ 5