BD FACSCelesta Multicolor Flow Cytometry Training

severe

Compensation beads saturate detectors causing overflow

Compensation bead populations appear off-scale or saturate detectors, producing signals outside the linear detection range and preventing accurate compensation matrix calculation.

💡 4 · ✓ 4

severe

Tandem dye degradation on compensation beads

Tandem fluorophore compensation beads show spectral shift or altered emission profile over time, causing incorrect spillover calculation and poor compensation in acceptor channels.

💡 4 · ✓ 5

severe

Poor Resolution of Dim Markers Masked by Autofluorescence

Low-expression markers become indistinguishable from background. Positive and negative populations show poor separation, with dim fluorophores completely masked by cellular autofluorescence.

💡 4 · ✓ 5

moderate

Poor Signal-to-Noise Ratio with Dim Fluorophores on Weak Markers

Weak or low-abundance markers show poor separation from negative populations and isotype controls, especially when conjugated to dim fluorophores, making population discrimination difficult.

💡 4 · ✓ 6