Qiagen RNA Extraction: Clean Results Protocol

severe

Low RNA Yield After Cleanup

Recovered RNA concentration is significantly lower than expected based on input amount. Spectrophotometric measurement shows inadequate RNA quantity for downstream applications.

💡 5 · ✓ 5

moderate

Unexpected or Incorrect Quantification Value

Qubit Fluorometer shows unexpected concentration values that do not match expected sample concentration

💡 10 · ✓ 10

minor

Sample Out of Range - Too High

Qubit Fluorometer screen indicates samples are OUT OF RANGE (too high), sample fluorescence exceeds highest standard

💡 3 · ✓ 5