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Invasion Assay

🚨 Failure Case Library (4) + Submit your own case

moderate
Too many migrated cells — packed solid, can't count
Lower surface is densely covered with cells that fuse together; individual cells cannot be counted.
💡 4 · ✓ 4
moderate
Migration is too slow — treatment and control look identical
After extended culture the wound has barely closed and the difference between groups is not visible.
💡 4 · ✓ 3
moderate
Staining is too light — cells hard to discern
Cells on the membrane are very faintly colored; impossible to count confidently or photograph clearly.
💡 5 · ✓ 4
moderate
Cells are unevenly distributed across the membrane — poor reproducibility
Different fields of view show wildly different cell density; replicate inserts disagree.
💡 4 · ✓ 4
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